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| Registros recuperados: 15 | |
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| Ribeiro,L.A.; Mariani,P.D.S.C.; Azevedo,J.L.; Rech,E.L.; Schmidt,G.S.; Coutinho,L.L.. |
| Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions obtained for high embryo survival and high transformation efficiency were achieved with 800 psi helium gas pressure, 500 mmHg vacuum, gold particles, an 8 cm DNA-coated microparticle flying distance to the embryo and embryo placement 0.5 cm from the center of the particle dispersion cone. Under these conditions, transformation efficiency was 100%, survival rate 25% and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biolistic process; Chicken embryo; Gene transfer; SS-galactosidase; Green fluorescent protein; GFP. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900003 |
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| Linden,Rafael; Matte,Ursula. |
| Gene therapy attempts the insertion and expression of exogenous genetic material in cells for therapeutic purposes. Conceived in the 1960s, gene therapy reached its first clinical trial at the end of the 1980s and by December 2013 around 600 genuine open clinical trials of gene therapy were registered at NIH Clinical Trials Database. Here, we summarize the current efforts towards the development of gene therapy in Latin America. Our survey shows that the number of scientists involved in the development of gene therapy and DNA vaccines in Latin America is still very low. Higher levels of investment in this technology are necessary to boost the advancement of innovation and intellectual property in this field in a way that would ease both the social and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene therapy; Gene transfer; Gene delivery; Viral vector; South America. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200015 |
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| Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Salvadori,José Roberto; Nicolau,Marcio; Kaltchuk-Santos,Eliane; Alves,Laura Bianchessi; Bodanese-Zanettini,Maria Helena. |
| The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anticarsia gemmatalis; Glycine max; Plant resistance to insects; Gene transfer. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700003 |
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| Tan,Dao Xuan; Tuong,Ho Manh; Thuy,Vu Thi Thu; Son,Le Van; Mau,Chu Hoang. |
| ABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S- GmDREB2- c-myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic tobacco lines... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drought-resistant; GmDREB2 gene; Gene transfer; Soybean; Transcription factors. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651 |
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| Okamoto,AS; Andreatti Filho,RL; Rocha,TS; Menconi,A; Marietto-Gonçalves,GA. |
| The expansion of global poultry production has increased the need to reduce or control the agents responsible for economic losses, including Salmonella spp. These bacteria are also of public health concern due to their potential to cause food poisoning, and, more recently, due to the antimicrobial resistance presented by these bacteria. Molecular biology is an important tool currently used in the diagnosis and research studies of main poultry diseases. The present studied analyzed 100 samples of Salmonella Enteritidis (SE) isolated from avian material aiming at detecting the class 1 integron gene, Integroninvolved in antimicrobial resistance, by means of polymerase chain reaction (PCR), and comparing it with plate inhibition test. Subsequently, SE samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Integron; Gene transfer; Resistance to antimicrobials; Salmonella Enteritidis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300009 |
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| RIOS,MARIANA; VENEGAS,ALEJANDRO; CROXATTO,HORACIO B. |
| Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (<A HREF="#rios97">Ríos et al., 1997</A>). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal... |
| Tipo: Journal article |
Palavras-chave: SS-galactosidase; Gene transfer; Oviduct. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007 |
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| Zhao,Qing; Jing,Ji; Wang,Gang; Wang,Jie Hua; Feng,Yuan Yuan; Xing,Han Wen; Guan,Chun Feng. |
| Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging. In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene. The possibility of facilitating the screening process for transgenic plants expressing functional proteins using gfp marker was explored. In order to realize high transformation efficiency, different explant sources including undifferentiated callus pieces and petioles were compared for their regeneration efficiency and susceptibility to... |
| Tipo: Journal article |
Palavras-chave: Cocultivation; Gene transfer; Green fluorescent protein; Regeneration. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500009 |
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| Cadoret, Jean-paul; Bardor, M; Lerouge, P; Cabigliera, M; Henriquez, Vitalia; Carlier, Aude. |
| Extraction of natural substances and chemical synthesis are the main sources of pharmaceutical molecules. When possible, one may transfer the gene of the molecule in living cells creating individual factories producing on demand and in a safe way the requested molecule. Today, bacteria, yeast, mammalian cells and plants constitute the main plate-forms for various commercial products. Microalgae present numerous advantages and could offer a powerful tool for the production of commercial molecules in a near future. |
| Tipo: Text |
Palavras-chave: Genetics; Recombinant; Algae; Pharmaceuticals; Gene transfer; Biotechnology; Génétique; Recombinant; Algues; Pharmaceutique; Transfert de gène; Biotechnologie. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4045.pdf |
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| Neumann,Alexander J; Alini,Mauro; Archer,Charles W; Stoddart,Martin J. |
| Background: Retroviral vectors are commonly used for gene transfer applications and they represent an effective way to provide a sustained delivery of a bioactive factor in basic research and tissue engineering applications. Cells that have been transduced with retroviral vectors ex vivo are usually amplified on tissue culture plastic, for a prolonged period of time, in order to obtain sufficient cell numbers prior to the experiment of interest. However, the effect of the transgene product on the transduced cells, during this period of time, is rarely, if ever, investigated. The current study investigated if transduction with a VSG.G pseudotyped retroviral vector expressing human bone morphogenetic protein 2 (Rv.BMP-2) influences the gene expression... |
| Tipo: Journal article |
Palavras-chave: Constitutive expression; Controls; Gene transfer; Monolayer expansion; Retrovirus. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200007 |
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| Dani,S.U.. |
| Gene therapy is the treatment of diseases based on the transfer of genetic information. Agents that carry or deliver DNA to target cells are called vectors (Latin vector: carrier, deliverer). Ideally, a vector should accommodate an unlimited amount of inserted DNA, lack the ability of autonomous replication of its own DNA, be easily manufactured, and be available in concentrated form. Secondly, it should have the ability to target specific cell types or to limit its gene expression to specific cell types, and to achieve sustained gene expression in the long term or in a controlled fashion. Finally, it should not be toxic or immunogenic. Such a vector does not exist and none of the DNA delivery systems so far available for in vivo gene transfer is perfect... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene therapy; Gene transfer; DNA delivery; Viral vectors; Diposomes; Vector development. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200001 |
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| Registros recuperados: 15 | |
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